Effect of Basic Fibroblast Growth Factor on Apoptosis of Scleral Cells in Lens-induced Myopia of Guinea Pig
- VernacularTitle:碱性成纤维细胞生长因子对豚鼠透镜诱导性近视眼巩膜细胞凋亡的影响
- Author:
Hong WANG
;
Lei GAO
;
Jia-Hui CAO
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Sclera;
Apoptosis;
Proliferation;
Basic fibroblast growth factor;
Lens induced myopia
- From:
Journal of Environment and Health
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of basic fibroblast growth factor(bFGF)on the apoptosis of scleral cells in the posterior pole in lens-induced myopia of guinea pigs and to discuss the mechanism of bFGF in inhibiting the formation of myopia. Methods Four-week-old cleaning healthy guinea pigs were randomly divided into the control group,LIM group,LIM+PBS group, LIM+the bFGF 100 ng group,LIM+the bFGF 500 ng group,LIM+the bFGF 1 000 ng group,15 in each.Except the control group, the right eye of guinea pig in other groups wore-10 D concave lens for 7 days and then different concentration of bFGF or PBS were injected into the vitreous cavity,3 days later injected again.After 15 days of-10 D concave lens treatment,the eyeballs were removed and the apoptotic cells in the scleras were determined by electron microscopy,TUNEL technique and flow cytometry.The proliferation of scleral cells in the posterior pole were determined by Ki-67 immune histochemistry stain.Results Compared with the control group,the significant differences were detected in the right eyes of LIM group(P
