Antagonistic Effect of N-acetylcysteine on Hepatic Mitochondria Damage Induced by Cadmium in Rats
- VernacularTitle:N-乙酰半胱氨酸对镉致大鼠肝线粒体损伤的影响
- Author:
Kun GUAN
;
Zhao-Fa XU
;
Fang-Lin ZHANG
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Cadmium;
N-acetylcysteine;
Mitochondria;
Liver
- From:
Journal of Environment and Health
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the antagonistic effect of N-acetylcysteine(NAC)on the damage of hepatic mitochondria of rats induced by cadmium in vitro.Methods The mitochondria were prepared from the clean Wistar rats' whole liver by using differ ential centfifugation.The mitochondria were incubated in the assay buffer containing different concentration of CdCl_2 (10,100,1 000,10 000 ?mol/L)at 37 ℃ for 1 h.The effect of NAC(500 ?mol/L)was studied at a CdCl_2 concentration of 1 000 ?mol/L.The incubation buffer was collected and the level of GSH,cytochrome C and the activity of Mn-SOD were determined. Results Compared with the control group,the level of GSH and Mn-SOD in 100,1 000,10 000 ?mol/L CdCl_2 groups were significantly decreased,the content of cytochrome C in 1 000,10 000 ?mol/L CdCl_2 groups were significantly increased(P
