Expression of Fas/FasL and the apoptosis of HepG2 cells transfected with LIGHT and IFN-?
- VernacularTitle:LIGHT/INF-?基因配比转染HepG2细胞对其凋亡及Fas/FasL系统表达的影响
- Author:
Zheng-Hua WANG
;
Li-Qun WU
;
Bing HAN
;
Yun LU
;
Zhen-Hua LV
;
Xiang-Ping LIU
;
Kun YANG
;
Ai-Hua SUI
;
Chong-Yao BI
;
- Publication Type:Journal Article
- Keywords:
Apoptosis?LIGHT?Interferon-??Fas/FasL?Transfection?HepG2 cell
- From:
Chinese Journal of Current Advances in General Surgery
1999;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of Fas、FasL and the apoptosis of liver cancer cell line HepG2 transfected with LIGHT and IFN-? gene mediated by Cationic liposome.Methods:HepG2 cells were divided into two groups(the solo transfection of LIGHT gene and the combined transfection of LIGHT and IFN-? genes) and the control groups(no transfection).HepG2 cells were cellected at 12h,24h and 48h after transfection.The apoptosis of HepG2 cells and the expression of Fas and FasL of the HepG2 cells were investigated with flow cytometry.Results:After transfection,the apoptosis of HepG2 cells increased,and the apoptosis of combined transfection group was higher than the solo transfection of LIGHT(P
