An oncosis-like cell death of pancreatic cancer Panc-1 cells induced by artesunate is related to generation of reactive oxygen species
- VernacularTitle:青蒿琥酯通过活性氧损伤机制诱导胰腺癌细胞发生胀亡性死亡
- Author:
Ji-Hui DU
;
Zhen-Jian MA
;
Jia-Xuan LI
;
Hou-De ZHANG
;
- Publication Type:Journal Article
- Keywords:
artesunate;
pancreatic cancer;
oncosis;
reactive oxygen species
- From:China Oncology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
50?mol/L induced markedly oncotic cell death,but no apoptosis was observed.TEM examination indicated that a form of cell death accompanied by cellular swelling,organelle swelling and vacuolization,mitochondrial swelling and cristae membrane loss,and nucleus swelling, chromatin scattering or karyolysis,which characterized as oncosis.When treated with 50,200?mol/L of ART for 5 h, the intracellular ROS level of Panc-1 cells markedly increased to 1.60 and 4.49 fold compared with that of untreated cells,respectively.Pretreatment with TCEP effectively attenuated ART-induced intracellular ROS level and decrease the oncosis in Panc-1 cells.Conclusions:ART exerts profound cytotoxic effects on Panc-1 cells and induces an oncosis- like cell death,which is quite different from apoptosis.The cellular generation of ROS and its peroxidation damage may be one of the mechanisms for its anti-tumor effect on pancreatic cancer.
