The role of GSK-3?in apoptosis induced by proteasome inhibitors combined with paclitaxel in ovarian cancer cells and its possible mechanism
- VernacularTitle:人卵巢癌细胞SKOV3对硼替佐米和紫杉醇联合应用的药物敏感性及可能机制
- Author:
Dan-Hui WENG
;
Xiao-Hong SONG
;
Fan-Fei KONG
;
Liang-Sheng FAN
;
Yan LI
;
Hui XING
;
Ding MA
;
Shi-Xuan WANG
;
- Publication Type:Journal Article
- Keywords:
proteasome inhibitors;
bortezomib;
GSK-3?;
paclitaxel;
chemoresistance;
apoptosis
- From:
China Oncology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:Proteasome inhibitors such as bortezomib,represent an interesting new class of potential anticancer drugs.In the present study,we explored the sensitivity of ovarian cancer cell line SKOV3 to paclitaxel,proteasome inhibitors and their combination,and also studied the involvement of GSK-3?/Mcl-1 signaling pathway in the regulation of apoptosis induced by those agent.Methods:Methyl thiazolyl tetrazolium (MTT)assay was applied to examine the cell viability,Annexin-V/PI apoptosis detection kit was used to determine the apoptosis rate of different groups,and western blot assay was introduced to evaluate the expression levels of phosphorylated GSK-3?and Mcl-1.Results:In the MTT assay,the cell viability ratios of combination group at serial time points from 12 to 72 hr were(65.2?5.8)%,(58.3?14.4)%,(35.3?5.0)%,(19.2?1.5)% and(11.4?2.5)%,and there were significant differences as compared to the treatment of paclitaxel alone(P
