Expression of tyrosine kinase receptor B in OVCAR-3 ovarian cancer cells and its significance
- VernacularTitle:酪氨酸激酶受体B在OVCAR-3卵巢癌细胞中的表达及意义
- Author:
Xiao-Hui YU
;
Bin CAI
;
Yi-Xia YANG
;
Qin YAN
;
Xiao-Ping WAN
;
- Publication Type:Journal Article
- Keywords:
TrkB;
BDNF;
ovarian cancer;
anoikis;
multicellular spheroids
- From:China Oncology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:TrkB,a neurotrophic tyrosine kinase receptor,serves as a potent and specific suppressor of anoikis of non-malignant epithelial cells,and induces high invasion capacity in these cells.TrkB over-expression has been associated with chemotherapy resistance and poor survival in neuroblastoma and some other highly aggressive cancers.However,the relationship of the expression of TrkB to anoikis resistance in ovarian cancer cells has rarely been reported in literature.This study investigated the expression and significance of anoikis- suppressor TrkB in OVCAR-3 ovarian cancer cells.Methods:The expression of TrkB and its ligand BDNF was evaluated in OVCAR-3 ovarian cancer cells under different culture conditions by RT-PCR and Western blot.Results: TrkB mRNA was overexpressed in multicellular spheroids(cell-spheroids,anchorage-independent culture,AIC)as compared to that in OVCAR-3 cells(adhesive-cells,adhesive culture,AC),(35.3?0.7)% versus(23.5?0.5)%;but BDNF mRNA expression was the opposite to the above situation,(41.4?0.6)% versus(32.24?0.7)%(P
