Promotion of invasion and metastasis in human breast carcinoma MCF-7 by SDF-1?
- VernacularTitle:基质细胞衍生因子-1?促进乳腺癌细胞侵袭和转移
- Author:
Xing HUA
;
Li FANG
;
Li-Na YU
;
- Publication Type:Journal Article
- Keywords:
SDF-1?;
breast carcinoma MCF-7 cells;
invasion;
metastasis
- From:
China Oncology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:We have already reported viral macrophage inflammatory protein-Ⅱcan induce surface chemokine receptor CXCR4 internalizated.Based on the diverse biological functions of SDF-1?/ CXCR4,this study was to investigate the effect of SDF-1?on invasion and metastasis of human breast carcinoma MCF-7 cells.Methods:MCF-7 cell's ability of invasion,metastasis and anoikis were used as end points.The invasive ability was measured by the number of cells that were able to penetrate polycarbonates coated with matrigel.The metastastatic ability was analyzed by Transwell chamber.The anoikis ability was detected by FCM.Results:SDF- 1?+MCF-7 cells formed long and abundant pseudopodia,and only few filopodia were detectable in MCF-7 cells.It was shown that adhesive and metastasis capability of MCF-7 cells was enhanced with SDF-1?cocultured(P
