Effect of Selenium on Expression of eNOS and iNOS Induced by Fluoride in ECV-304
- VernacularTitle:硒对氟诱导人脐静脉血管内皮细胞iNOS和eNOS表达的影响
- Author:
Xiao-Dong HOU
;
Xin-Ying LIN
;
Jian-Chao BIAN
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
FluorideY SeleniumY Nitric oxide synthaseY Inducible nitric oxide synthase
- From:
Journal of Environment and Health
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To knowthe effect of selenium on expression ofnitric oxide synthase(eNOS) and inducible nitric oxide synthase(iNOS) induced by fluoride in ECV-304(human umbilical vein endothelial cells).Methods The cells,ECV-304,were divided into two groups and treated as follows,one group was treated with sodium fluoride at different doses(0,400,1 000 and 2 000 ?mol/L),the other was treated with sodium fluoride at different doses(0,400,1 000 and 2 000 ?mol/L)and added sodiumselenite(100 nmol/L) respectively.24 h of treatment,The activity ofeNOS and iNOS were measured by NO and NOS assay kits and the expression of eNOS mRNA and iNOS mRNA was analyzed by RT-PCR.Results The activity of eNOS and the expression of eNOS mRNA were down-regulated by fluoride with a dose dependent manner,while the activity of iNOS and the expression of iNOS mRNA were up-regulated.Selenium showed a significant antagonism to low level fluoride treated groups.Conclusion Seleniumcan antagonize the effect offluoride on the expression ofiNOSand eNOS in the vein endothelial cells when the fluoride exposure level is not toohigh.
