Determination of acyclovir and gancyclovir using flow-injection chemiluminescence method
- VernacularTitle:流动注射-化学发光法测定阿昔洛韦和更昔洛韦
- Author:
Abudukeremu MUNIRA
;
Nannan WANG
;
Yuhai TANG
;
MHETA'ER TU'ERHONG
;
- Publication Type:Journal Article
- Keywords:
flow-injection;
chemiluminescence;
Ce(IV);
rhodamine B;
acyclovir;
gancyclovir
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a new flow-injection chemiluminescence(FI-CL) method for the determination of acyclovir and gancyclovir by using the CL system of Ce(IV)-Rhodamine B.Methods The redoxreaction of Ce(IV) and acyclovir/gancyclovir in H2SO4 medium could generate CL signal.Rhodamine B could obviously sensitize this signal,and the CL intensity was proportional to the concentration of acyclovir and gancyclovir.Therefore,a new FI-CL method has been described for the determination of acyclovir and gancyclovir.Results For acyclovir,the determination range was 3.0?10-5g/L-7.0?10-2g/L,with 1.56?10-5g/L as its determination limit.During 11 repeated measurements for 1.0?10-3g/L acyclovir,the relative standard deviation was 2.08%.For ganciclovir,the determination range was 5.0?10-5g/L-7.0?10-2g/L,with 2.35?10-5g/L as its determination limit.The relative standard deviation was 2.83%,with 11 repeated measurements of 1.0?10-3g/L ganciclovir.Conclusion This method has broad linear range,high sensitivity,and convenience and speediness,it therefore,can be successfully used to determine the content of ganciclovir in injections.
