Staurospolin promotes the apoptosis of multidrug resistance tumor cell lines induced by adriamycin
- VernacularTitle:十字孢碱促进多柔比星诱导的多药抗药性肿瘤细胞系的凋亡
- Author:
Shi-Yong WANG
;
Wei-Li DU
;
Zhen-Jun YANG
;
Huan YU
;
Yun-Feng YANG
;
Hui ZHANG
;
- Publication Type:Journal Article
- Keywords:
Drug resistance,multidrug;
Protein kinase C;
Enzyme inhibitors;
Apoptosis;
Carcinoma
- From:
Cancer Research and Clinic
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether the protein kinase C inhibitor can promote the apopto- sis of multidrug resistance tumor cell lines which are induced by chemotherapy drugs.Methods Choose the KB/S(oral squamous cancer cell line)and KB/VCR(its multidrug resistant cell line)to compare the Adri- amycin-induced apoptosis with or without staurospolin(protein kinase C inhibitor).The apoptosis is stained with acridine orange,tested by flow cytometry,and approved by electron microscope.Results 36 hours after the treatment with 0.04 ?g/ml adriamycin,apoptotic cells of KB/S are 96.68%,and after 48 hours,the apop- totic cells of KB/VCR are 64.99%.When the concentration of adriamycin are augmented to 0.4?g/ml and 2.0?g/ml,the apoptotic cells of KB/VCR are 69.74% and 37.18% respectively.When treated with stau- rospolin together,the apoptotic cells of KB/VCR increased to 72.58%(?~2=4.5,P0.05)respectively.These results were testified by electron microscope and acridine orange-stain.Conclu- sion The resistance to apoptosis may be one of the mechanisms of multidrug resistance and the protein ki- nase C inhibitor may reverse this resistance by promoting the apoptosis of multidrug resistance tumor cells.
