A sandwich method of enzyme immunoassay for serum FT_4
- VernacularTitle:血清游离甲状腺素夹心酶联免疫分析法
- Author:
Hong ZHENG
;
Xue-Qin ZHAO
;
Jing-Yu ZHANG
;
- Publication Type:Journal Article
- Keywords:
Small molecular hapten;
Two-site system;
Sandwich immunoenzymometric assay;
Thyroxin
- From:
Chinese Journal of Endocrinology and Metabolism
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
T_4 was coupled to human serum albumin(HSA)to make the conjugate T_4-HSA,a polyvalent T_4 analogue rather than monovalent T_4 per se alone.A new strategy for sandwich enzyme immunoassay model system of small molecular hapten hormones FT_4 was developed,such as intra-assay CV,inter-assay CV,average recovery, normal and abnormal values were fit for clinical application.The sensitivity was as much as 10 times higher than conventional competitive enzyme immunoassay.
