Effects of Smad7 on transdifferentiation and collagen I synthesis in AGE-stimulated NRK52E cells
- VernacularTitle:Smad7对终末期糖基化产物介导大鼠肾小管上皮细胞转分化及Ⅰ型胶原合成的影响
- Author:
Liao SUN
;
Hui-Li SUN
;
Xiao-Yan LI
;
Xiang-Yang WANG
;
Wen-Fang CHEN
;
Ning LUO
;
Yan-Yan SUN
;
Xiu-Qing DONG
;
Xue-Qing YU
;
- Publication Type:Journal Article
- Keywords:
Glycosylation end products;
advanced;
Smad 7;
Transdifferentiation;
Collagen typeⅠ
- From:
Chinese Journal of Endocrinology and Metabolism
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of smad7 on transdifferentiation and collagenⅠsynthesis in advanced glyeosylation end-products(AGE)-stimulated NRK52E cells.Methods NRK52E cells were transferred by pTet-on plasmid system and the cell lines of doxycycline(Dox)-regulated Smad7 expression were selected for the study.Transnuclear location of p-Smad2/3 was examined with immunocytochemistry.The mRNA and protein expressions of Smad7,?-SMA,E-cadherin,collagenⅠwere detected with RT-PCR and Western blot. Results AGE-induced expressions of Smad7 mRNA and protein were further increased in NRK52E cells by the addition of Dox in a dose-dependent manner.Overexpression of Smad7 caused a marked inhibition of p-Smad2/3 transnuclear location at 30 min(68.3% vs 31.2%,P
