Therapeutic effect of neuropeptide PACAP27 on Parkinson's disease in mice
- VernacularTitle:垂体腺苷酸环化酶激活肽27治疗帕金森病小鼠的实验研究
- Author:
Gang WANG
;
Yu-Yan TAN
;
Xiao-Kang SUN
;
Ru-Jing REN
;
Hai-Yan ZHOU
;
Sheng-Di CHEN
;
- Publication Type:Journal Article
- Keywords:
Parkinson disease;
Pituitary adenylate cyclase-activating polypeptide;
1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine
- From:
Chinese Journal of Neurology
2005;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different doses of pituitary adenylate cyclase- activating polypeptide(PACAP)on the functional and morphological outcome in a mice model of Parkinson' s disease(PD)rendered by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP).Methods Male mice were treated with PACAP 0.02, 0.20 or 2.00 ?g by iv bolus for 7 days after MPTP was administered, and were compared with the saline-treated mice.The immunohistochemistry and Western blot were used to detect the alterations of PD biomarker including tyrosine hydroxylase(TH), dopamine transporter(DAT)and vesicular monoamine transporter2(VAMT2).In addition, monoamine neurotransmitters in the striatum of mice were measured by the high performance liquid chromatography (HPLC).Results TH immunohistochemistry indicated that the number of TH-positive neurons in the substantia nigra was increased in all PACAP-treated mice(PACAP(0.02 ?g/d)group was 93.33?4.87, F=85.85,P
