The experimental study on the relationship between the hepatic glycogen content and the liver transplantation of non-heart-beating donor
- VernacularTitle:增加供肝的肝糖原贮备减轻心脏停搏大鼠供肝的热缺血再灌注损伤
- Author:
Tang-Lei SHAO
;
Wei-Ping YANG
;
Wei-Yao CAI
;
- Publication Type:Journal Article
- Keywords:
Heart arrest;
Donor selection;
Liver glycogen;
Liver transplantation;
Reperfusion injury;
Rat
- From:
Chinese Journal of Organ Transplantation
2005;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study whether the warm ischemia-reperfusion injury can be alleviated if the hepatic glycogen content were increased before liver transplantation.Methods The male SD rats as donors and recipients were divided into groups A,B and C randomly.In group A,the rats were al- lowed access to solid and water ad libitum;In group B,the rats drank glucose liquor for 4 days prior to liver harvesting;In group C,the rats were intravenously injected with 50% glucose besides the protocol in group B 3-4 h prior to liver harvesting.According to the non-heart-beating time(the non- heart-beating time were set into 60,90,120 and 150 min),the rats in groups A,B and C were divided into 4 subgroups,and the orthotopic liver transplantation was performed and the hepatic glycogen and ATP contents were measured.The one-week survival rate and the serum levels of MDA and SOD in the recipients were determined after liver transplantation.Results The hepatic glycogen and ATP con- tents in groups B and C were more than those in group A significantly(P
