Expression of raf-1 in HepG2 strain stably transfected by HBx gene
- VernacularTitle:稳定转染HBx基因的HepG2细胞对raf-1表达的影响
- Author:
Peng-Fei CAO
;
Yong-He LIU
;
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
X protein;
Cell cycle;
raf-1 protein
- From:
Cancer Research and Clinic
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect on cell cycle of hepatitis B virus x protein and ap- proach the molecular mechanism of x protein's carcinogenesis. Methods Gene transfection mediated by the lipofectamine was used to introduce the eukaryotic expression vector of HBV x gene into human hepato- cellular carcinoma cell line HepG2(HepG2X cell).The selective medium containing G418 was used to select the cell clones which the X protein was expressed constantly.Flow eytometry was used to detect the cycle of the cells;raf-1 protein was detected by Western blot.Results X protein could be expressed on 21?10~3 loca- tion in the transfected cells,while there were no protein expressed in the control cells.The proliferation of X cells increased significantly according to MTS method(P
