E1AF gene expression and its clinical significance in human rectal cancer
- VernacularTitle:实时荧光定量RT-PCR分析E1AF在直肠癌中的表达及其临床意义
- Author:
Hai-Yi LIU
;
Bing XU
;
Yu-Jian ZENG
;
Jun-Min SONG
;
Yong-Yang YU
;
Zong-Guang ZHOU
;
Hong-Zhi LUO
;
Lie YANG
;
Ling WANG
;
Bin ZHOU
- Publication Type:Journal Article
- Keywords:
Rectal neoplasms;
E1AF;
Reverse transcriptase polymerase chain reaction(RT-PCR
- From:
Cancer Research and Clinic
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect EIA factor expression in human rectal cancer and normal tissue and to determine whether it is correlated with invasion and metastasis of human rectal cancer. Methods Real- time RT-PCR was used to detect E1AF expression in matched rectal cancers and normal tissues from g6 in- patients.Results Among the 86 rectal cancer samples tested,55 cases E1AF mRNA overexpression was ob- served. The mRNA expression of E1AF in the sample group was remarkably different from that in the control group.In carcinomas,E1AF mRNA expression correlated significantly with histological type,depth of inva- sion,lymph node and distant metastasis and advanced Duke stage.Conclusion E1AF is correlated signifi- cantly with invasion and metastasis of human rectal cancer and may be an important factor in the invasion and metastasis.
