Establishment of lymphatic metastasis model for human gastric cancer in nude mice
- VernacularTitle:人类胃癌裸鼠淋巴结转移模型的建立
- Author:
Jin-Sheng WU
;
Zhi-Gang WANG
;
Sha MI
;
- Publication Type:Journal Article
- Keywords:
Gastric carcinoma;
Lymphatic metastasis;
Mice,nude;
Models,animal;
Neo-plasm transplantaion
- From:
Cancer Research and Clinic
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the method of establishing lymph node metastasis model for gastric cancer in nude mice.Methods Human poorly differentiated gastric cancer SGC-7901 ceils were cul- tured,inherited,amplified and collected in vitro,and were inoculated in nude mice subcu.taneously.After pas- saging six generations,the subcutaneous tumor was orthotopically implanted in nude mice.The mice were then sacrificed on the 9th week after implantation.Serum CEA density was detected using radio-immunity method. Orthotopically implanted tumors,remote organ metastases as well as lymph node metastasis were observed macrescopically and histologically.Results The successful rate of orthotopically transplantion of gastric can- cer was 100 %.The rate of perigastric lymph node metastases was 93.3 %.Local infiltration and remote metastasis were observed.The level of serum CEA in nude mice bearing tumor was significantly higher than that of normal nude mice(P
