Study on the relationship among the NF-?B activation,uPA expression and apoptosis induced by 5- Fu in HCT116 human carcinoma cell line
- VernacularTitle:5-氟尿嘧啶诱导结肠癌细胞凋亡与NF-kB活化及uPA表达的关系
- Author:
Jian-Ye LI
;
Yuan-Man REN
;
Wen-Gang PENG
;
Chun-Fang LIN
;
Yong-Gang ZHANG
;
- Publication Type:Journal Article
- Keywords:
Colon neoplasms;
5-fluorouracil;
Apoptosis;
HCT116cells;
NF-?B;
uPA;
PDTC
- From:
Cancer Research and Clinic
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship among apoptosis,NF-KB activation and uPA expres- sion in human colon carcinoma cell line HCTll6 induced by 5-fluorouracil,and to observe the effect of in- hibiting activity of NF-KB by PDTC on apoptosis as well as expression of uPA.Methods Cell apoptosis was analysed by Annexin V-FITC.Fluctuation of NF-KB and uPA was detected by semi-quantitative immuno- histochemistry.Results 5-fluorouracil could induce apoptosis and activate NF-KB.PDTC could significantly increase the apoptosis and suppress the activation of NF-KB induced by 5-fluorouracil.There was a positive correlation between the changes of uPA and NF-KB.Conclusion 5-fluorouracil could induce apoptosis,ac- tivate NF-KB and up-regulate expression of uPA of HCT116 cells.The mechanism of enhanced apoptosis by PDTC may be related to suppressing activation of NF-?B and down-regulating expression of uPA.
