Effects of long-term ethanol intake on testosterone synthesis and androgen binding protein mRNA expression in rat testis
- VernacularTitle:长期饮酒对大鼠睾丸睾酮合成和雄激素结合蛋白mRNA表达的影响
- Author:
Ming-Feng CAO
;
Jin-Jiao JIANG
;
Qiang WAN
;
Ling GAO
;
Yi LIU
;
Jia-Jun ZHAO
;
- Publication Type:Journal Article
- Keywords:
Alcohol;
Receptors,benzodiazepine;
Androgen-binding protein;
Testosterone;
Rats,Wistar
- From:
Chinese Journal of Endocrinology and Metabolism
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different doses of alcohol on the synthesis of testosterone and the expression of androgen binding protein(ABP)mRNA in rat testis.Methods Forty male Wistar rats were randomly divided into 4 groups(10 rats each group)and received either distilled water(control group)or alcohol(alcohol-fed groups)for 5 months.Alcohol was administered by garage with a single daily dose : 5 g/kg(large dose group),2.5 g/kg(middle dose group)and 0.5 g/kg(small dose group).Testosterone content was measured by ELISA.mRNA levels of peripheral-type benzodiazepine receptors(PBR),PPARct and ABP were assayed by RT-PCR.Results Compared with control group:(1)ethanol feeding with daily doses of 5 g/kg,2.5 g/kg and 0.5 g/kg significantly decreased testosterone levels by 31.13%(P0.05)respectively,indicating that ethanol might impair testosterone synthesis;(2) mRNA levels of PBR were decreased in all three ethanol-treated groups(all P
