Increased expression of protein tyrosine phosphatase 1B in pancreas of high fat diet-induced obese rats
- VernacularTitle:高脂饮食诱导的肥胖大鼠胰腺蛋白酪氨酸磷酸酶1B表达增高
- Author:
Li-Juan ZHANG
;
Da-Jin ZOU
;
Ping GU
;
Jie WU
;
Zheng ZHANG
;
- Publication Type:Journal Article
- Keywords:
Protein-tyrosine phosphatase 1 B;
Pancreas;
Insulin resistance
- From:
Chinese Journal of Endocrinology and Metabolism
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of protein tyrosine phosphatase(PTP)1B in pancreas of obese rats induced by high fat diet.Methods Twenty male SD rats were randomly divided into control group on regular diet(n=10),and obesity model group on high fat diet(n=10).After twelve weeks,fasting serum insulin,blood glucose,triglyceride,total cholesterol,epididymal fat weight were measured and the histomorphological change in liver were observed;glucose tolerance test and insulin releasing test were performed; The protein expression of PTP-1B in pancreas of rats was determined with Western blotting and immunohistochemistry.The phosphorylation of insulin receptor substrate-1(IRS-1)and insulin receptor(IR)were detected by immuno-precipitation.Results(1)The insulin sensitive index was significantly decreased in the high-fat-diet rats compared with the normal rats(0.36?0.18 vs 0.91?0.28,P
