Effect of estrogen receptor ? knocked down by RNA interference on osteoprotegerin expression in estrogeninduced human osteoblast-like cells
- VernacularTitle:RNA干扰基因敲低雌激素受体?对雌激素诱导人成骨样细胞护骨素表达的影响
- Author:
Zhao-Zhong WU
;
Min LIU
;
Jian-Qiang FENG
;
Wei LIN
;
Hao ZHANG
;
Chang-Wei WU
;
Jin-Yu WANG
;
- Publication Type:Journal Article
- Keywords:
RNA interference;
Osteoprotegerin;
Estrogen receptor?;
Human osteoblast-like cells
- From:
Chinese Journal of Endocrinology and Metabolism
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
After estrogen receptor?(ER?)in MC,63 cell was knocked down by RNA interference, expression of osteoprotegerin(OPG)induced by 17?-estradiol was assayed by RT-PCR.17?-estradiol with various concentration obviously upregulated the expression of OPG mRNA of MG63 cell with the maxima]effect at the concentration 10~(-7)mol/L,which was not inhibited by suramin(G protein inhibitor).The present result suggested that ER?was not involved in regulation of OPG mRNA expression in MC,63 cell by 17?-estradiol.
