Establishment of perfusion technique for isolated rat pancreas
- VernacularTitle:大鼠胰腺离体灌注技术的建立
- Author:
Nai-Qian ZHAO
;
Ye-Rong YU
;
Hui-Wen TAN
;
Zhi-Ming LU
;
Xiang-Xun ZHANG
;
Jun-Jie LI
;
- Publication Type:Journal Article
- Keywords:
Isolated perfused rat pancreas
- From:
Chinese Journal of Endocrinology and Metabolism
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an isolated rat pancreas perfusion technique,a method for the precise measurement of insulin secretion in vitro.Methods An isolated rat pancreas perfusion technique was applied in the study of insulin secretion from?-cells in 10 high-fat diet-induced obese Wistar rats.Results For the assessment of the functional integrity of the perfused pancreas,the isolated pancreas of 6 rats met all the criteria: (1)The constancy of perfusion pressure was kept over the whole experiment time[(70?5)mm Hg,1 mm Hg= 0.133 kPa].(2)The duodenal peristaltic activity of isolated pancreas and duodenum block was present after perfusion experiment.(3)Total insulin response to arginine stimulation was significantly increased as compared with glucose stimulation[maximum insulin secretion rate:(987?100)?U/min vs(545?50)?U/min,P
