Effect of removed epineurium of peripheral nerve and transplanted into spinal cord injury in rats
- VernacularTitle:去除外膜的外周神经移植对大鼠脊髓损伤的影响
- Author:
Wen-Qian MA
;
Shao-Cheng ZHANG
;
Yong-Bi YAN
;
Can-Rong NI
;
- Publication Type:Journal Article
- Keywords:
Spinal cord;
Injury;
Microsurgical technique;
Peripheral nerve;
Graft
- From:
Chinese Journal of Microsurgery
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the nerve recanalization and the motor function of hind legs after transplantation of peripheral nerve grafts treated with microsurgical technique at chronic spinal cord injury (SCI) in rats.Methods The SD rats were established into SCI model with improved Allen method.The rats were divided into two parts 12 weeks after the injury.In experimental group:by microsurgieal technique. the sural nerves were removed epineufium and transplanted into SCI lesion,control group rats were treated without any operation.Retrograde HRP tracing through sciatic nerve were practiced at 1 month,2 month,3 month after transplantation of peripheral nerve grafts.The morphological changes were observed at section of spinal cord and the motor functions of both hind legs of rat were detected.Results The morphology of the injured spinal cord sections turned better.Retrograde HRP tracing through sciatic nerve showed some HRP positive markers at the site of near rostral end of the nearly injured part at one month after transplantation and increased with the time going by.Motor function of hind legs of rats recovered significantly in transplantation groups.Conclusion Peripheral nerve grafts treated with mierosurgical technique have repairing effect on chronic spinal cord injury in rats.
