Calbindin-28 in rats of Parkinson's disease after deep brain stimulation to subthaiamus nucleus
- VernacularTitle:深部脑刺激丘脑底核治疗帕金森病大鼠脑中钙调蛋白的变化
- Author:
Chun-Yan CAO
;
Zhen-Guo LIU
;
Sheng-Di CHEN
;
Bo-Min SUN
;
Shi-Kun ZHAN
;
Guo-Qiang LU
;
- Publication Type:Journal Article
- Keywords:
Parkinson disease;
Electric stimulation therapy;
Subthalamus nucleus;
Calciumbinding proteins;
Synaptophysin
- From:
Chinese Journal of Neurology
2005;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of deep brain stimulation(DBS)therapy to Parkinson's disease(PD).Methods We produced hemi-parkinsonian rat model with stereotaxically injecting 6-OHDA to right medial forebrain bundle(MFB)and stimulated ipsilateral subthalamu nucleus (STN)with platinum electrodes chronically to investigate the influence of DBS to the expression of Calbindin-28,synaptophysin and tyrosine dioxydase(TH)in Striatum by Western blot.In addition,slices of bilateral PD rats after DBS were stained to observe the expression of Calbindin-28 and synaptophysin in substantia nigra by Immunohistochemistry.Results High frequency stimulation impaired the rotational frequency 31% of unilateral PD rats triggered by apomophine;Long-term DBS increased the expression of TH in innocent striatum of unilateral PD rats 78.6%?9.5%,since the ipsilateral striatum(lesion side) was TH depleted by 6-OHDA insults;Calbindin-28 expression in ipsilateral striatum of hemi-PD rats raised up 75.4%?15.0% and long-term DBS reduced the effect by 43.0%?7.1%,meanwhile Calbindin-28 positive neurons in substantia nigra compacta in sham,PD and DBS rats were 74.5?10.2,75.7?15.6, 33.1?7.8.However,Synaptophysin expression in substantia nigra and striatum kept stable even after long- term DBS.Conclusions Consistent to the treatment to PD patients,DBS to STN alleviated the motor disorder of PD rats,the treatment might be based on regulating the expression of Calbindin-28 and TH.
