Construction and identification of RhoA specific siRNA expression vector in hepatocellular carcinoma cell
- VernacularTitle:肝癌细胞中RhoA小干扰RNA载体的构建与鉴定
- Author:
Yong-Qing LU
;
Ji-Rong SU
;
Hong-Lin DONG
;
Shan LU
;
Wen-Long LI
;
Wei WU
;
Zhi-Jun ZHANG
;
Jiu-Chi LI
;
- Publication Type:Journal Article
- Keywords:
Liver neoplasms,Experimental;
Genetic vectors;
RNA interference
- From:
Cancer Research and Clinic
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct RhoA siRNA plasmid expression vector.Methods According to the computer aided design,RhoA-specific siRNA gene was synthesized and cloned into the RNAi-Ready Pgenesil-1 Vector.The constructed RhoA-RNAi plasmid were transfected into human HEPG2 cell.Western blot was used to detect the effect of RhoA-RNAi plasmid.Results The recombinant was cloned and the se- quence was obtained.RhoA-RNAi plasmid can down-regulate the expression of RhoA in human hepatocel- lular carcinoma cell line HEPG2.Conclusion Successfully cloning the recombinant makes it possible for searching new mechanism of RhoA in hepatocellular carcinoma.
