Relationship between protein level and ? mRNA,? mRNA isoforms of glucocorticoid receptor and effect of intravenous injection of methylprednisolone effect in multiple sclerosis patients
- VernacularTitle:多发性硬化患者糖皮质激素受体蛋白和mRNA表达与激素疗效的相关性
- Author:
Xueqiang HU
;
Zhi-Yin LOU
;
Guo-Yu ZHOU
;
- Publication Type:Journal Article
- Keywords:
Multiple sclerosis;
Receptor,glucocorticoid;
Methylprednisolone;
Radioligand assay;
Reverse transcriptase-polymerase chain reaction
- From:
Chinese Journal of Neurology
2005;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of glucocorticoid receptor(GR), GR? mRNA and GR? mRNA in peripheral blood mononuclear cells(PBMC)of patients with multiple sclerosis(MS) and healthy controls and investigate the relationship between GR protein, GR? mRNA, GR? mRNA and intravenous injection methylprednisolone(IVMP)effect.Methods GR in PBMC was measured by radioligand assays in 20 patients with relapsing-remitting MS(RRMS), 6 with the second progressive MS (SPMS)and 26 healthy controls.Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR)was used to detect the expression of GR? mRNA and GR? mRNA in PBMC of patients with MS and healthy controls.The effect of IVMP was evaluated by the expanded disability status scale(EDSS). Results(1)Before IVMP, GR in PBMC of MS was significantly lower(RRMS:(3.8?0.2)?10~3 site/cell;SPMS:(1.6?0.2)?10~3 site/cell)than those of controls((4.2?0.8)?10~3 site/cell, P
