Effects of cell cycle inhibitor on astroglial proliferation and scar formation and neuroprotection after focal cerebral ischemia in rats
- VernacularTitle:细胞周期抑制剂对大鼠局灶性脑缺血后胶质细胞增殖和瘢痕形成的影响及对神经元的保护作用
- Author:
Gui-Bin ZHANG
;
Yun-Lan XU
;
Xiang LI
;
Ping WANG
;
Dai-Shi TIAN
;
Yi-Xing DU
;
Min-Jie XIE
;
Wei WANG
;
- Publication Type:Journal Article
- Keywords:
Brain ischemia;
Astrocytes;
Cell division;
Kinetins;
Cyclins;
Apoptosis
- From:
Chinese Journal of Neurology
2005;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of cell cycle inhibitor on astrocytic proliferation and scar formation and to study neuronal apoptosis after focal cerebral ischemia in rats.Methods Ischemic model was established by photochemistry method.T_2-weighted MRI was performed on the 3rd, 7th, and 30th day after focal cerebral ischemia.The expression of glial fibrillary acidic protein(GFAP)and apoptosis was observed by immunofluorescence.The protein levels of GFAP and proliferation cell nuclear antigen (PCNA), CyelinA and CyclinB1 were measured by Western blotting from the ischemic and sham animals finished on the 3rd, 7th, and 30th day.Results A marked and significant reduction of brain infarction volume was found in Olomoucine-treated ischemic animals(2.27%?0.28% , 1.87%?0.19%, 1.08%? 0.18%)as compared with controls(5.10%?0.35%, 4.60%?0.26%, 3.96%?0.28%, P
