Effect of transplantation of embryonic stem cell-derived cardiomyocytes on cardiac function of rats with myocardial infarction
- VernacularTitle:由胚胎干细胞分化的心肌细胞移植对心肌梗死大鼠左心室重构及心功能的影响
- Author:
Xin-Chun YANG
;
Fang-Fang YI
;
Jun CAI
;
- Publication Type:Journal Article
- Keywords:
Cell transplantation;
Stem cells;
Myocardial infarction;
Ventricular remodeling;
Ventricular function,left
- From:
Chinese Journal of Organ Transplantation
2005;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether transplantation of embryonic stem cell-derived cardiac myocytes (ESCMs) directly into the infarcted myocardium could improve the cardiac function in the rats.Methods Cell culture medium with or without ESCMs was injected into the borders of car- diac scar tissue one week after experimental infarction.Cardiac performance was evaluated 4 weeks later by means of echocardiography after ESCMs (n=16) or medium (n=12) injection.Results Im- munostaining confirmed the presence of?-actinin,?-MHC,and cTnI in the beating EBs.RT-PCR analysis of EBs revealed the expression of cardiac-specific genes,including cardiac Nkx 2.5,GATA-4 and?-MHC.ESCMs implantation significantly improved fractional shortening compared with medium- treated hearts (P
