Changes and significance of serum nitric oxide levels in the early stage after rat liver transplantation
- VernacularTitle:大鼠肝移植后早期血清一氧化氮的变化及其意义
- Author:
Bing TU
;
Yong PENG
;
Yi YAN
;
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Nitric oxide;
Reperfusion injury;
Rats
- From:
Chinese Journal of Organ Transplantation
2005;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes and significance of serum nitric oxide levels in the early stage after rat liver transplantation.Methods Male Sprague Dawley rats were used as do- nors and recipients of orthotopic liver transplantation,and the time of cold preservation and anhepatic phase was 4 h and 25 min respectively.Forty-eight rats were randomly divided into groups A,B and C (n=16 each).The samples of blood were taken from vena cava and hepatic tissues from left lobe 2 h, 4 h,6 h in groups A,B and C respectively after liver graft reperfusion.ALT and NO levels in serum were detected,and the expression of NF-?B in hepatic tissue was examined by immunohistochemical technique.Amounts of bile flow in 5 min after liver graft reperfused initially were measured.The pathological changes in liver tissues were observed.Results Amounts of bile flow in 5 min in group A (3.73?1.11?l) were greater than those group B (2.35?0.92?l) and group C (2.23?0.81?l) (P
