Study on microsatemte instability of p14,p15,p16 gene in gastric carcinoma
- VernacularTitle:胃癌中p14、p15、p16基因微卫星不稳定性的研究
- Author:
Jin-Yu PANG
;
Xuan-Qin YANG
;
Su-Hong LI
;
Li LI
;
Quan-Hong WANG
;
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Adenocarcinoma;
High-grade intraepithelial neoplasia;
Microsatellite instability;
Gene,p14,p15,p16
- From:
Cancer Research and Clinic
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the microsatellite instability (MSI) of D9S166,D9S171,D9S941, D9S942 and IFNA located at chromosome 9p21 in gastric carcinoma with high-grade intraepithelial neoplasia and normal tissue,and investigate the correlation of p14,p15,p16 gene and the gastric carcinogenesis. Methods 55 cases of gastric carcinoma with high-grade intraepithelial neoplasia and normal tissue were se- lected to detect MSI by microdissection,polymerase chain reaction,denaturing polyaerylamide gel elec- trophoresis and silver nitrate staining technology of five microsatellite markers on 9p21.Results In the infor- mative cases,total ratio of MSI in gastric carcinoma was 27 % (64/233) and it was 18% (42/233) in high- grade intraepithelial neoplasia.The ratio of MSI was significantly higher in gastric carcinoma than that in high-grade intraepithelial neoplasia (P
