The sequence of fat deposit and its relationship with insulin resistance
- VernacularTitle:脂肪分存的顺序及其与胰岛素抵抗的关系
- Author:
Xiu-Ping BAI
;
Hong-Liang LI
;
Wen-Ying YANG
;
Jian-Zhong XIAO
;
Bing WANG
;
Rui-Qin DU
;
Da-Jun LOU
;
- Publication Type:Journal Article
- Keywords:
Fat deposit;
Insulin resistance;
Gene expression
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the sequence of fat deposit and its relationship with insulin resistance in SD rats fed by high fat diet.Methods Normal 8-week-old male SD rats were randomly divided into normal chow (NC,n=40)and high fat diet(HF,n=40)groups.Triglyceride(TG)in serum,liver and muscle were measured;glucose infusion rate(GIR)and the mRNA level of genes related to lipid metabolism in liver and muscle were determined in different stages.GIR was detected by eugiyeemic-hyperinsulinemic clamp for evaluating the insulin sensitivity.Gene expression was determined by real-time PCR.Results(1)As compared with NC group,serum TG was not increased after high fat feeding for4 and 8 weeks,it began to increase after 12 weeks [0.52(0.15-1.00) mmol/L vs O.31(0.09-0.53)retool/L, P0.05)in skeletal muscle.After 8 weeks,the expression of ACC1 in liver in HF group was increased by 20.6%,CPT-1 was decreased by 27.1%(P
