The Inhibitory effect of dendritic cells modified by sCD40 on lymphocytes phenotypes and cytokines production
- VernacularTitle:sCD40基因修饰树突状细胞抑制T细胞表型及细胞因子分泌
- Author:
Jie-Feng HE
;
Hao-Liang ZHAO
;
Sheng-Li DONG
;
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Antigens;
CI40;
Immune tolerance;
Cytokines
- From:
Chinese Journal of Organ Transplantation
2005;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibitory effects of dendritic cells modified by sCD41)gene on T lymphocytes phenotypes and cytokines production and the mechanism of inducing donor-specific immune tolerance in vitro.Methods T lymphocytes prepared with Nylon Fiber Column from Balb/c mice(as reaction cells)and DCs of different groups(as stimulation cells)were subjected to primary mixed lymphocyte culture(MLC).After incubation for 7 days,the responsiveness of the cells was de- tected by MTS method at the indicated time points,and supernatants were assayed for IFN-?,IL-2, IL-4,IL-10 by ELISA kits.On the day 5,the cultured cells were assessed for the expression of CD4, CDS,CD25 and CD69 by using flow cytometry(FCM).After secondary MLC for 5 days,the same indexes were assayed hy using the same methods.Results Dendritic cells modified by sCD40 could in- duce the hyporesponsiveness to alloantigen in primary and secondary MLC.In primary MLC,the ex- pression of CD4~+ and CD8~+ T cells and CD4~+ CD25~+,CD8~+ CD25~+,CD4~+ CD69~+,CD8~+ CD69~+ T cells in IX;modified by sCD40 group were less than those in control group(P
