Protective effect of bFGF gene modified mesenchymal stem cells on cerebral ischemia in rats
- VernacularTitle:bFGF基因修饰的间充质干细胞对大鼠脑缺血模型的保护作用
- Author:
Jie YANG
;
Fu-Feng ZHANG
;
Wen-Ping GU
;
Bo XIAO
;
Bei-Sha TANG
;
Qi-Dong YANG
;
- Publication Type:Journal Article
- Keywords:
Brain ischemia;
Mesenchymal stem cell transplantation;
Fibroblast growth factor 2;
Rats
- From:
Chinese Journal of Neurology
2005;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of basic fibroblast growth factor(bFGF) gene modified mesenchymal stem cells(MSCs-bFGF)on cerebral isehemia in rats.Methods MSCs or MSCs-bFGF were transplanted into rat models of focal cerebral ischemia by intravenous injection.The neurological deficits and infarction volumes were evaluated,and the survival rate and differentiation of grafted MSCs were observed by double immunofiuoreseent labeling.Results In the rat cerebral ischemic model, both MSCs and MSCs-bFGF showed protective effect on the rats in comparison with control group.However, the protective effect was more significant in MSCs-bFGF group.Double immunofluorescent staining showed the number of BrdU-labeled and NeuN co-expression cells in MSCs-bFGF treated animals(127.40?7.43 and 11.20?3.09)were much more than in those of MSCs treated animals.While there was no significant difference between MSCs-bFGF and MSCs group in the number of GFAP co-expression cells.Conclusion MSCs transplantation has protective effect on cerebral ischemia in rats.Basic fibroblast growth factor gene modified MSCs is more effective than MSCs in neuroproteetion.
