Differentiation of human embryonic stem cells into neural stem cells in vitro
- VernacularTitle:体外诱导人类胚胎干细胞定向分化为神经干细胞
- Author:
Feng-Guang LI
;
Hui-Fang XU
;
Hong CHEN
;
Jing-Yan CHAI
;
Bian-Zhi XING
;
Yan-Qiang ZHAN
;
Xiao-Qing LI
;
Shou-Gang GUO
;
Su-Ming ZHANG
;
- Publication Type:Journal Article
- Keywords:
Neurons;
Stem cells;
Cell differentiation;
Embryonic induction
- From:
Chinese Journal of Neurology
2005;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore a kind of simple and high efficient approach to differentiate human embryonic stem cells(hESCs)into neural stem cells(NSCs).Methods hESCs were cultured in bacterial culture dish filled with serum free medium to gain embryoid bodies.Then the mature embryoid bodies grew in the special medium including B27 and noggin by adherent culture to differentiate into NSCs. Results The hESCs kept floating in the bacterial culture dish and growing all the time and then formed mature embryoid bodies 7 to 10 days later.The embryoid bodies could be differentiated into highly pure (96.4%)nestin positive cells.And these cells were differentiated into all kinds of neural cells if cultured further.Conclusions This kind of method is less time-consuming,cheaper,and more efficient than those of the results in literatures reported.It affords very good source of seed cells for cell transplantation therapy in the future.
