Antiproliferation and induction differentiation of rosiglitazone in human gastric carcinoma transplanted into nude mice
- VernacularTitle:罗格列酮对裸小鼠胃癌移植瘤细胞的抗增生及诱导分化作用
- Author:
Fang-Zhi CHEN
;
Li-Hui ZHU
;
Jian-Feng HU
;
Li ZHANG
;
Guo-Qing LI
;
Lan WEN
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Rosiglitazone;
Cell proliferation;
Enzyme induction
- From:
Cancer Research and Clinic
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antiproliferation and induction differentiation of human gas- tric carcinoma which human gastric lower-differentiation mucinous carcinoma MGC-803 cells transplanted in- to nude mice by using rosiglitazone(ROS),and to preliminarily explore the mechanism of differentiation. Methods The mice were randomly divided into five groups:model,ATRA,ROS 25 mg?kg~(-1),ROS 50 mg/kg, ROS 100 mg/kg.After that the volumes were measured and inhibition rates were calculated.The cell cycle was detected by FCM.The protein expression level of Mucin SAC was detected by immunohistochemistry. Results The volume of tumor decreased significantly in ROS treatment groups,the differences had statistical significance compared with model group(P0.05).The xenograft tumors of ROS groups demonstrated the characteristics of differentiation.Xenograft tumor cells were arrested in G_0/G_1 phase,and the cells in S phase decreased significantly,and up-regulated Mucin SAC gene expression.Conclusion ROS could inhibit the growth of tumor,and the effect were dose-dependent with ROS.ROS could induce the differentiation of Xenograft tumor cells of gastric cancer.Its mechanism might be related to the inhibit of transition from G_1 to S phase,degrade the activity of proliferation,regulate the expres- sion of Mucin 5AC.
