Detection of micrometastasis in perioperative primary esophageal cancer patients using real-time quantitative reverse transcrtption-PCR
- VernacularTitle:食管癌围手术期外周血微转移的定量检测及其临床意义
- Author:
Ming JIANG
;
Zhi-An LIU
;
Jian-Hua ZHAO
;
Lin XU
;
- Publication Type:Journal Article
- Keywords:
Esophageal neoplasms;
Neoplasm metastasis;
Carcinoembryonic antigen;
Surgical manipulation
- From:
Cancer Research and Clinic
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a desirable quantitative assay system to evaluate the correlation between surgical manipulation and micrometastasis in primary esophageal cancer patients.Methods 118 pe- ripheral blood samples from 40 esophageal cancer patients undergoing radical resection were collected before surgery(B-1),immediately after surgery(B0)and at the third day postoperatively(B+3).Based on real-time quantitative reverse transcription-PCR,carcinoembryonic antigen(CEA)mRNA gene was used in the detec- tion.12 patients with benign tumor and 13 healthy volunteers were collected as negative control groups.Re- sults The median of CEA mRNA copies at B-1,B0 and B+3 were 1592,13 314 and 6221 copies/ml blood, respectively.CEA mRNA expression levels were found to be significantly higher at both B0 and B+3 than that of B-1(P=0.0001 and 0.0209,respectively).No significant difference was found between B0 and B+3(P= 0.4396).Conclusion Surgical manipulation on esophageal cancer patients increases the probability of mi- crometastasis.Therefore,adjuvant therapy is needed during perioperative stage.
