Total flavonoids of Herba Epimedii increases Cbfal expression in bone of ovariectomized rats
- VernacularTitle:淫羊藿总黄酮促进去卵巢大鼠骨组织Cbfal表达
- Author:
Xiu-Zhen ZHANG
;
Guo-Feng QIAN
;
Hong LI
;
Zhi-Gang ZHU
;
- Publication Type:Journal Article
- Keywords:
Epimedium brevicornum;
Flavones;
Osteoporosis;
Core binding factor?1;
Fluorescence quantitative PCR
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of total flavonoids of Herba Epimodii (HEF) on the expression of core binding factor?1 (Cbfal) in the bone of ovariectomized rats.Methods Fifty-four female SD rats were allocated into 6 groups (9 in each): sham operation group,ovariectomized (OVX) group,OVX followed by three kinds of HEF doses(40,80 and 160 mg?kg~(-1)?d~(-1))and nilestriol (0.1 mg?kg~(-1)?week~(-1))for 12 weeks respectively.Bone mineral density (BMD) of whole body was determined by DEXA.The levels of osteocalcin (BGP) and estradiol (E_2) in serum were measured by radioimmunologic method.All rats were then sacrificed,and total RNA were directly isolated from the skull.Relative quantification of Cbfal mRNA expression was detected by real time quantitative PCR.Results Serum E_2 and BMD of whole body in the OVX group were lower than those in sham group significantly after 12 weeks (both P0.05).Relative quantification of Cbfal mRNA expression in the OVX group was significantly lower than that in sham group after 12 weeks (P
