Emodin stimulates glucose uptake by HepG2 hepatocyte through activation of PPAR?
- VernacularTitle:大黄素通过激活PPAR?促进HepG2细胞葡萄糖摄取
- Author:
Li-Juan YANG
;
Hai-Yan YU
;
Yi-Ming MU
;
Bao-An WANG
;
Jing-Tao DOU
;
Ju-Ming LU
;
Chang-Yu PAN
- Publication Type:Journal Article
- Keywords:
Emodin;
PPAR?;
HepG2 hepatocytes;
Glucose uptake rate;
Glucose transport protein-2
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct PPAR?and PPAR?response element (PPRE)-controlled luciferase expression vectors,and to determine whether the traditional Chinese medicine emodin activates PPAR?and improves the glucose uptake by HepG2 hepatocytes.Methods (1) PPAR?and PPRE luciferase expression vectors were constructed and were applied to screen more than 20 ingredients of the traditional Chinese medicine. (2) HepG2 cells were incubated with emodin which can activate PPAR?and PPRE luciferase activity,and the PPAR?mRNA expression level was evaluated by RT-PCR/Southern blot.(3) PPAR?and glucose transporter 2 (Glut2) proteins were determined by Western blot analysis in HepG2 cells treated with emodin.(4) The glucose uptake rate was measured using 2-deoxy-[~3H]-D-glucose in HepG2 cells after treatment with emodin.Results (1) Emodin stimulated luciferase activity controlled by PPRE in dose-dependent manner at concentrations of 0.04 to 180?mol/L in COS-7 cells.The highest value was about 4 folds of control in the cells treated with 90?mol/L emodin (P
