Effects of tumor necrosis factor-?and rosiglitazone on the expression of protein tyrosine phosphatase 1B during the differentiation of 3T3-L1 preadipocytes
- VernacularTitle:肿瘤坏死因子?和罗格列酮对3T3-L1细胞分化过程中蛋白酪氨酸磷酸酶1B表达的影响
- Author:
Yue CHEN
;
Da-Jin ZOU
;
Zheng ZHANG
;
Miao WANG
;
Jie WU
;
- Publication Type:Journal Article
- Keywords:
Protein tyrosine phosphatase 1B;
Tumor necrosis factor-?;
Rosiglitazone;
3T3-L1 preadipocytes;
Adipocyte differentiation
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protein level of protein tyrosine phosphatase 1B (PTP1 B) during the differentiation of 3T3-L1 preadipocytes and the effects of tumor necrosis factor-?(TNF-?) and rosiglitazone on the expression of PTP1B during the differentiation,and to explore the relationship between PTP1B and adipecyte differentiation.Methods 3T3-LI preadipecytes were cultured in vitro and differentiated by three groups of inducers: basic inducers only (3-isobutyl-1-methylxanthine+dexamethasone+insulin,group C),20?g/L TNF-?+basic inducers (group CT) and 10~(-5) mol/L rosiglitazone+basic inducers (group CR).Protein level of PTP-1B in adipocytes during differentiation was detected by Western blot.Results Each group showed the relatively high level of PTP1B in 3T3-L1 preadipecytes,going down with the differentiation of adipecytes,and reaching the lowest level in fully-matured adipecytes.Comparing the late period of differentiation in these three groups,CT group was sluggishly differentiated with more PTP1B protein,and CR group showed active differentiation with the lowest level of PTP1B.Conclusion PTP1B decreases with the differentiation of adipoeytes.The effects of TNF-?and rosiglitazone on insulin sensitivity perhaps partly via their influences on PTP1B level in adipecytes.
