Differentiation of neural stem cells transplanted into ventral horn of spinal cord after brachial plexus avulsion
- VernacularTitle:脊髓源性神经干细胞在臂丛撕脱伤后相应前角内分化情况观察
- Author:
Zhi-Yuan TU
;
Wen-Ming ZHANG
;
Wei-Qin ZHU
;
Zhi-Hong ZHENG
;
Jian-Shi HU
;
- Publication Type:Journal Article
- Keywords:
Brachial plexus;
Nerve root avulsions;
Neural stem cells;
Ventral horn,Spinal cord;
Transplantation
- From:
Chinese Journal of Microsurgery
2000;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the survival,migration and differentiation of the neural stem cells which transplanted into ventral horn of spinal cord after brachial plexus avulsion.Methods Neural stem ceils isolated from spinal cord of neogenetic rats and cultured,expanded,labeled by BrdU before transplanted. Twenty adult healthy SD rats preformed as the model of brochial plexus avulsion(Roots C_(5～7)),then transplan- rod stem ceils into the C_6 ventral horn of spinal cord.On 1,2,4,8,12 weeks postoperatively,immunohisto- chemistry assay were carried out in the spinal cord.Results Transplanted into ventral horn of spinal cord after brachial plexus avulsion.Neural stem cells can survive,migrate for at least one segment of spinal cord and differentiate to neurons and astrocytes.The differentiation of stem cells were time-depends.Conclusion Neural stem cells can survive,migrate and differentiate after transplanted into ventral horn of spinal cord in the rats which suffered from brachial plexus avulsion.
