Study on apoptosis of human ovarian cancer cell induced by siRNA on Rab25
- VernacularTitle:Rab25基因siRNA诱导人类卵巢癌细胞A2780凋亡的实验研究
- Author:
Yang FAN
;
Xiao-Yan XIN
;
Bi-Liang CHEN
;
Xiang-Dong MA
;
Li SHANG
;
- Publication Type:Journal Article
- Keywords:
Ovarian neoplasms;
Gene,Rab25;
RNA,small interfering;
Cell growth;
Cell proliferation;
Cell apoptosis
- From:
Cancer Research and Clinic
1999;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of siRNA of Rab25 on apoptosis induction in ovarian carci- noma cell.Methods According to Rab25 mRNA sequence in the Genebank,the plasmids expressing siRNA against Rab25 were constructed and stably transected into A2780 cells,MTY method were applied to measure cell growth and proliferation.Apoptosis rate and cell cycle phase distribution of A2780 cells were measured by flow cytometry(FCM).Apoptosis was confirmed by agarose gel electrophoresis(AGE)of DNA.Results Cells transected with the plasmids expressing siRNA targeting Rab25 gene effectively decreased cell growth, proliferation;blocked A2780 cells in the G_1 phase of cell cycle and induced cell apoptosis.A typical DNA ladder pattern appeared on AGE.Conclusion Rab25 gene siRNA can inhibit growth and induce apoptosis in ovarian carcinoma cell line,which will facilitate further studies of Rab25 function and its application in the treatment of ovarian cancer.
