Expression of cyclin dependent kinases-5 in the temporal lobes of the drug resistance epilepsy patients
- VernacularTitle:耐药性癫癎患者颞叶中细胞周期依赖性蛋白激酶5的表达
- Author:
Zhi-Qin XI
;
Xue-Feng WANG
;
Zhen-Li GUO
;
Xian-Jun KE
;
Ji-Jun SUN
;
Yuan WU
;
Jin-Mei LI
;
Fei XIAO
;
Xi ZHU
;
Li-Feng GUAN
;
Yun GONG
;
Fengying LIU
;
Guoming LUAN
;
Jianguo ZHANG
;
Yuping WANG
;
- Publication Type:Journal Article
- Keywords:
Epilepsy;
Temporal lobe;
Cyclin-dependent kinases;
Drug resistance
- From:
Chinese Journal of Neurology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of cyclin dependent kinases 5(CDK5)in the temporal lobes of the epilepsy patients and to explore the possible roles of CDK5 in the pathogenesis of refractory epilepsy.Methods The brain tissues of intractable epilepsy(IE)were studied by fluorescence quantative polymerase chain reaction(FQ-PCR)for CDK5 mRNA,while immunohistochemistry and Western blot were used to study the protein expression.Nonepileptogenic control brain tissues were used for comparison.Results FQ-PCR analysis showed that the expression of CDK5 mRNA in epilepsy patients was significant higher than those in the control group.And immunohistochemistry showed that the protein mainly existed in the neuron and glial.At the 35000 relative molecular mass,Western blot could been seen that there is a limpid strap.The optical density of CDK5 in IE(temporal lobe 1.4293?0.1839,hippocampus 2.0733?0.4738)was significantly higher than that in the control(temporal lobe 0.9680?0.4147, hippocampus 1.4030?0.6160,P
