Influence of estradiol benzoate on induction of the model of systemic lupus erythematosus in BALB/c mouse
- VernacularTitle:苯甲酸雌二醇对BALB/c小鼠系统性红斑狼疮模型诱导的影响
- Author:
Xiao-Yun TANG
;
Chang-Long LV
;
Xiao-Li ZHANG
;
Xia LI
;
- Publication Type:Journal Article
- Keywords:
Estradiol;
Systemic lupus erythematosus(SLE);
Antinuclear antibody(ANA);
Anti-histone antibody(AHA);
Lupus nephritis(LN)
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the influence of estradiol benzoate in induction of BALB/c mouse systemic lupus erythematosus model. Methods: Gonadectomied BALB/c mice were immunized with homologous spleen cell activated by concanavalin A three times,and at the same time injected with different doses estradial benzoate. Serum antinuclear antibody, antihistone antibody and estradiol in serum and kidney tissue homogenate were detected by enzyme linked immunosorbent assay. Kidney lesions were detected by hematoxylin-eosine(HE) and periodic acid-Schiff(PAS) staining, and by transmission electron microscope for detecting electron-dense agent as well as by direct fluorometric method for detecting immunoglobulin G antigen-antibody complex. Results: BALB/c mice immunized with homologous spleen cells activated by concanavalin A all developed systemic lupus erythematosus, but a statistically significant variation was observed in both autoantibody production and kidney damage were observed with a statistically significant variatron in the groups receiving different dosage of estradiol benzoate group(P
