TGF-?_1 induced the tolerant dendritic cells and the mechanism
- VernacularTitle:转化生长因子?_1诱导的耐受性树突状细胞及其机制
- Author:
Zheng-Rong LIU
;
Wei-Ming LI
;
Min ZHANG
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Transforming growth factor beta;
Immune tolerance
- From:
Chinese Journal of Organ Transplantation
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the induction of tolerant dendritic cells(DOs)and its mechanism in mice.Methods The dendritic cell line derived from C57BL/6 mouse.DO2.4 cells were co-cultured with TGF-?_1(20 ng/ml)to induce the tolerant DCs(TGF?-DC)and stimulated wihh li- popolysaccharide(LPS)for 48 h to induce the mature DCs(LPS-DC).Special small interference RNA molecule(siRNA)of PIR-B was chemically synthesized and was transfected into TGF?-DC by lip2000 (si-DC).The expression of paired immunoglobin receptor A/B(PIR-A/B)in DC2.4 cells was mea- sured by semi-quantitative RT-PCR and flow cytometry(FCM).The allogeneic stimulating capacity of DCs was measured by mixed lymphocyte reaction(MLR)using ~3H-thymidine incorporation test with the Balb/c spleen cells as reaction cells.The concentration of IFN-?in supernatants of MLR from dis- tinct groups was tested by enzyme linked immunosorbent assay(ELISA).Results TGF-?_1 up-regula- ted the PIR-B mRNA expression and down-regulated the PIR-A mRNA expression(P
