PKB-dependent pathway in colorectal cancer cell growth induction by bFGF
- VernacularTitle:PKB介导的bFGF在人类大肠癌细胞中的表达及其相关性
- Author:
Jie LIN
;
Ji-Xian SHAN
;
- Publication Type:Journal Article
- Keywords:
Fibroblast growth factorz;
Protein kinase B;
Intestinal neoplasms
- From:
Cancer Research and Clinic
1997;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the signal transduction of bFGF to regulate the colorectal carci- noma cell growth via PI3K/PKB pathway.Methods MTT assay was used to determine the suppressive effect of bFGF on the growth rate of LoVo cell;(?-~(32)P)ATP incorporation assay was used to detect the increased activity of PKB in LoVo cell;the RT-PCR technique was used to test the expression of cyclin A;Western Blot analysis was used to detect the expressions of PKB and cyclin A protein.Results When the bFGF worked on the LoVo cell at various times,it was found that the PKB activity of the LoVo cell was increased by the(?-~(32)P) ATP incorporation assay.By preprocessing by the depressor-LY294002 and bFGF,the activity of PKB was sig- nificantly reduced(P
