Significance of detecting the mutations of K-ras gene in faecal for diagnosis colorectal carcinoma
- VernacularTitle:粪便中K-ras基因突变检测在大肠癌诊断中的意义
- Author:
Chen MA
;
Le-Ming ZHANG
;
Xiao-Gang CHEN
;
Gang YE
;
Lei FANG
;
- Publication Type:Journal Article
- Keywords:
Feces;
Genes,ras;
Intestinal neoplasms;
Diagnosis
- From:
Cancer Research and Clinic
1997;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the significance of detecting the mutations of K-ras gene in faecal for diagnosis colorectal carcinoma.Methods 36 cases suffering from colorectal carcinoma,24 cases from colorectal benign diseases and 24 cases from healthy subjects,all cases had been diagnosed by enteroscope. K-ras gene mutation at the codon 12 was detected by PCR-RFLP,DNA was taken from faecal in modified method.Results K-ras mutation rate in colorectal carcinoma was 77.8 %,colorectal benign diseases was 25.0 %,healthy cases was 8.3 %.Conclusion Detecting the mutations of K-ras gene in faecalmay improve the rate of diagnosing colorectal carcinoma.
