Suppression of Mdr1 gene using RNA interference in cultured rat astrocytes induced by coriaria lactone
- VernacularTitle:RNA干扰抑制马桑内酯诱导的大鼠星形胶质细胞Mdr1基因的表达
- Author:
Tian-Hua YANG
;
Xin-Wang CHENG
;
Lin-Yu TIAN
;
Jia GENG
;
Lei CHEN
;
Dong ZHOU
;
- Publication Type:Journal Article
- Keywords:
P-glycoprotein;
Epilepsy;
Astrocytes;
Lactones;
RNA,small interfering
- From:
Chinese Journal of Neurology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To reverse P-glycoprotein-mediated muhiple drug resistance using RNA interference(RNAi)in cultured rat astrocytes.Methods Astroeytes overexpressing P-glyeoprotein induced by coriaria lactone were transfected with the short-hairpin RNA expression vector-p-SIREN shuttle designed to target Mdrl mRNA.The mRNA level of Mdrl gene was evaluated by real-time PCR;the P-glycoprotein was examined by immunocytochemistry and image analysis,meanwhile the rhodamine efflux was assessed by flow cytometry.Results The astrocyte model overexpressing P-glycoprotein were established and successfully transfected with the short-hairpin RNA expression vector-p-SIREN shuttle.The mRNA level of Mdrl gene was knocked down by 67.70%(P
