Changes of Sema3A and Np1 in the entorhinal cortex and dentate gyrus of temporal lobe epilepsy rat
- VernacularTitle:颞叶癫癎大鼠模型内嗅皮质和齿状回内Sema3A及其受体Np1的表达变化
- Author:
Xue-Qian YUAN
;
Bo XIAO
;
Tie-Yu TANG
;
Ling LI
;
Shu-Yu LI
;
Guo-Liang LI
;
- Publication Type:Journal Article
- Keywords:
Epilepsy,temporal lobe;
Olfactory pathways;
Dentate gyrus;
Mossy fibers,hippocampal;
Semaphorin-3A
- From:
Chinese Journal of Neurology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes of Sema3A and it′s receptor Npl in temporal lobe epilepsy(TLE)rat brain and the roles in epileptogenesis mechanism.Methods TLE model was established with male healthy SD rats,in which mossy fiber sprouting(MFS)was verified using Neo-Timm staining method.Sema3A mRNA,Npl mRNA and protein was respectively analyzed by immunohistochemistry and in situ hybridization in the entorhinal cortex(EC)or dentate gyrus(DG)at different time after LiCL-PILO induced TLE.Results There were Mossy fiber sprouting(7d:0.70?0.42,15d:1.50?0.52,30 d:2.20 ?0.41,60 d:2.50?0.51)in DG inner molecular layer(IML)of TLE rat compared with those of controls (P
