Antioxidant response element activator protects motor neurons from selected death
- VernacularTitle:抗氧化反应元件活化剂对选择性运动神经元损伤的保护作用
- Author:
Hui BU
;
Chun-Yan LI
;
Xiao-Yun LIU
;
Zhe LI
;
Bin LI
;
Meng-Meng SUN
- Publication Type:Journal Article
- Keywords:
Amyotrophic lateral sclerosis;
Excitatory amino acid antagonists;
Oxidative stress;
Antioxidants
- From:
Chinese Journal of Neurology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of antioxidant response element (ARE) activator- 5,6-dihydrocyclopenta[ C ]-1,2-dithiole-3-thione (CPDT) on organotypic spinal cord cultures and to study whether this activation can protect motor neurons from oxidative stress.Methods Organotypic spinal cord cultures were prepared using lumbar spinal cord slices from 8-day-old rat.Threo-hydroxyaspartate (THA) was continuously added into the culture medium for 3 weeks,which caused selective motor neuron death. Thus,the in vitro model of amyotrophic Lateral sclerosis (ALS) was established.15,30 ?mol/L of CPDT were added into the culture medium respectively.Ventral motor neurons survival was evaluated by immunohistochemical staining with monoclonal antibody SMI-32,a nonphosphorylated neurofilament marker. Ultrastructure was observed with electronic microscope.Results The pretreatment of organotypic spinal cord cultures with different concentrations of CPDT significantly increase the total number of ventral motor neurons (15?mol/L:(15.81?6.97) perexplant;30?mol/L:(16.25?6.74) perexplant respectively) compared with THA group ((5.31?5.76) perexplant) and the former had plentiful neurite extensions (n= 15,P
