Role of bone marrow mobilization in the angiogenesis in ischemic hindlimbs of diabetic mice
- VernacularTitle:骨髓动员在糖尿病小鼠缺血肢体新生血管形成中的作用
- Author:
Nan ZHANG
;
Hong-Kun ZHANG
;
- Publication Type:Journal Article
- Keywords:
Bone marrow mobilization;
Limb isehemia;
Diabetes;
experimental;
Angiogenesis
- From:
Chinese Journal of Endocrinology and Metabolism
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the angiogenesis effect induced by hone marrow mobilization on the ischemic hindlimbs of diabetic mice.Methods Diabetes mellitus was induced in mice by injection of streptozotocin.To observe the angiogenesis effect in ischemic limbs,unilateral femoral artery and all the side branches were excised and then was given granulocyte-colony stimulating factor (G-CSF) for bone marrow mobilization.Results The level of nitric oxide (NO) in plasma was significantly lowered 8 weeks after establishment of diabetes mellitus.After mobilization for 8 days,CD_(34)~+ cells in peripheral blood of mice were increased significantly [from (0.15?0.05)% to (2.30?0.41 )%],the vascular endothelial growth factor (VEGF) mRNA level of ischemic limb,which was examined with semi-quantitative RT-PCR,became higher in diabetic mice than that in non-diabetic mice.Two weeks following mobilization,the blood perfusion detected by LASER Doppler perfusion scan in the ischemic hindlimbs was significantly higher in the diabetic mice than in that the nondiabetic mice [(76.37?6.10) % vs (18.07?3.40) %,P＜0.05],vWF immunohistochemical staining showed that the density of capillaries was significantly greater in the mobilization group than that in the non- mobilization group [ (26.6?4.8 vs 11.5?2.6 )/per field,P＜0.05 ].Conclusion Diabetes mellitus reduced the modulatory role of the endothelium in mice,but therapeutic angiogenesis induced by bone marrow mobilization could be an effective treatment for ischemic limb disease in diabetic mice.
